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fluo 3 am ester  (Biotium)


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    Biotium fluo 3 am ester
    Fluo 3 Am Ester, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 508 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluo+3+am+ester/pm41687940-71-0-12?v=Biotium
    Average 95 stars, based on 508 article reviews
    fluo 3 am ester - by Bioz Stars, 2026-08
    95/100 stars

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    MIT and CMIT/MIT‐mixture (Kathon) cause a TRPA1‐mediated increase in the intracellular Ca 2+ levels in TRPA1‐transfected HEK293 cells. The cells were loaded with <t>Fluo</t> 3 and preincubated for 30 min with a TRPA1‐antagonist (10 μM A‐967079 (A96) or 200 μM HC‐030031 (HC)) or vehicle solution before the measurement of intracellular calcium was started. The background was measured for 15 s. Then, MIT, CMIT/MIT mixture water solution (Kathon) or AITC was added, and the measurement was continued for another 30 s. In HEK293 cells transfected with human TRPA1 (hTRPA1), MIT (A and B) and CMIT/MIT‐mixture (Kathon; D and E) induced a dose responsive increase in intracellular Ca 2+ levels, which was blocked by the TRPA1 antagonist A‐967079 (A96) (A and B). MIT also induced a TRPA1‐dependent Ca 2+ increase in mouse Trpa1 (mTRPA1)‐transfected cells (C). The results are presented as mean ± SEM of the area under curve n = 5; a representative experiment is shown out of 2–3 with similar results. The statistical analysis was done using two‐way anova and Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01 and *** p < 0.001; ns, not significant. In (B and E), the Hill slopes were fit based on the results to estimate EC 50 .
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    MIT and CMIT/MIT‐mixture (Kathon) cause a TRPA1‐mediated increase in the intracellular Ca 2+ levels in TRPA1‐transfected HEK293 cells. The cells were loaded with <t>Fluo</t> 3 and preincubated for 30 min with a TRPA1‐antagonist (10 μM A‐967079 (A96) or 200 μM HC‐030031 (HC)) or vehicle solution before the measurement of intracellular calcium was started. The background was measured for 15 s. Then, MIT, CMIT/MIT mixture water solution (Kathon) or AITC was added, and the measurement was continued for another 30 s. In HEK293 cells transfected with human TRPA1 (hTRPA1), MIT (A and B) and CMIT/MIT‐mixture (Kathon; D and E) induced a dose responsive increase in intracellular Ca 2+ levels, which was blocked by the TRPA1 antagonist A‐967079 (A96) (A and B). MIT also induced a TRPA1‐dependent Ca 2+ increase in mouse Trpa1 (mTRPA1)‐transfected cells (C). The results are presented as mean ± SEM of the area under curve n = 5; a representative experiment is shown out of 2–3 with similar results. The statistical analysis was done using two‐way anova and Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01 and *** p < 0.001; ns, not significant. In (B and E), the Hill slopes were fit based on the results to estimate EC 50 .
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    MIT and CMIT/MIT‐mixture (Kathon) cause a TRPA1‐mediated increase in the intracellular Ca 2+ levels in TRPA1‐transfected HEK293 cells. The cells were loaded with Fluo 3 and preincubated for 30 min with a TRPA1‐antagonist (10 μM A‐967079 (A96) or 200 μM HC‐030031 (HC)) or vehicle solution before the measurement of intracellular calcium was started. The background was measured for 15 s. Then, MIT, CMIT/MIT mixture water solution (Kathon) or AITC was added, and the measurement was continued for another 30 s. In HEK293 cells transfected with human TRPA1 (hTRPA1), MIT (A and B) and CMIT/MIT‐mixture (Kathon; D and E) induced a dose responsive increase in intracellular Ca 2+ levels, which was blocked by the TRPA1 antagonist A‐967079 (A96) (A and B). MIT also induced a TRPA1‐dependent Ca 2+ increase in mouse Trpa1 (mTRPA1)‐transfected cells (C). The results are presented as mean ± SEM of the area under curve n = 5; a representative experiment is shown out of 2–3 with similar results. The statistical analysis was done using two‐way anova and Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01 and *** p < 0.001; ns, not significant. In (B and E), the Hill slopes were fit based on the results to estimate EC 50 .

    Journal: Pharmacology Research & Perspectives

    Article Title: The Contact Allergen Methylisothiazolinone ( MIT ) is a Potent Activator of the TRPA1 Ion Channel

    doi: 10.1002/prp2.70053

    Figure Lengend Snippet: MIT and CMIT/MIT‐mixture (Kathon) cause a TRPA1‐mediated increase in the intracellular Ca 2+ levels in TRPA1‐transfected HEK293 cells. The cells were loaded with Fluo 3 and preincubated for 30 min with a TRPA1‐antagonist (10 μM A‐967079 (A96) or 200 μM HC‐030031 (HC)) or vehicle solution before the measurement of intracellular calcium was started. The background was measured for 15 s. Then, MIT, CMIT/MIT mixture water solution (Kathon) or AITC was added, and the measurement was continued for another 30 s. In HEK293 cells transfected with human TRPA1 (hTRPA1), MIT (A and B) and CMIT/MIT‐mixture (Kathon; D and E) induced a dose responsive increase in intracellular Ca 2+ levels, which was blocked by the TRPA1 antagonist A‐967079 (A96) (A and B). MIT also induced a TRPA1‐dependent Ca 2+ increase in mouse Trpa1 (mTRPA1)‐transfected cells (C). The results are presented as mean ± SEM of the area under curve n = 5; a representative experiment is shown out of 2–3 with similar results. The statistical analysis was done using two‐way anova and Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01 and *** p < 0.001; ns, not significant. In (B and E), the Hill slopes were fit based on the results to estimate EC 50 .

    Article Snippet: The transfected HEK 293 cells were loaded with Fluo 3‐AM ester [2.5 μM Fluo 3‐AM in Hanks' Basic Salt Solution (Lonza), pH 7.45, with 25 mM 4‐(2‐hydroxyethyl)‐1‐piperazineethenesulfonic acid, 1 mg/mL BSA, 2.5 mM probenecid, and 0.08% Pluronic F‐127] for 40 min at room temperature.

    Techniques: Transfection